Microfluidic CD 4 + and CD 8 + T Lymphocyte Counters for Point - of - Care HIV Diagnostics Using Whole Blood Nicholas

clinical utility. device's resource-limited settings. Nevertheless, more testing on patients over time will be necessary to determine the and costing less per test than certain flow cytometers, it is possible that this device will be useful in −− analysis sample preparation, purification, and −− complete picture of infection. By integrating all steps of POC detection Unique to this device is the ability to monitor not only CD4 cells but also CD8 T cells, which can give a more T cells, thus suggesting that it is a viable platform for tracking HIV infection. + and CD8 + Watkins and colleagues found that their differential T cell counter worked as well as flow cytometry in counting CD4 from HIV-infected donors and healthy volunteers were tested and compared to the gold standard, flow cytometry. and a final count at the end, the authors were able to quantify the number of T cells in the sample. Blood samples either CD4 or CD8 T cells. Thus, by obtaining a total cell count at the beginning −− to capture the population of choice from the sample, leaving primarily white blood cells (including T cells). Antibodies decorated the microfluidic channels, the pore. The device also integrated sample preparation and cell selection. Red blood cells were lysed and removed how many cells traveled through et al. the current and causes a ''spike'' in signal. The number of spikes told Watkins flowed through a tiny pore that has a current passing through it; the cell, which doesn't conduct electricity, then blocks The microfluidic differential T cell counter is based on the Coulter counter principle: In the device, cells are incorporates all steps of sample preparation and accurate T cell counting. created a microfluidic chip for these point-of-care (POC) settings, which et al. Sub-Saharan Africa, so Watkins system and HIV infection. Current methods of counting aren't always available in resource-poor settings, such as The amount of CD4 and CD8 T cells in a blood sample can tell a doctor the status of the patient's immune Tiny T Cell Counter for HIV